Proteospin Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit

Product Description

• Columns bind proteins of interest while endotoxins flow through
• Reduce endotoxin levels to less than 0.1 EU/µg of protein
• Greater than 95% protein recovery
• High protein recovery with excellent endotoxin reduction
• Concentrate protein samples and remove detergents, salts and denaturants at the same time
• Effectively remove a wide range of detergents including SDS, Triton X-100^®, CHAPS, NP-40, and Tween 20
• Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix
• Also available as Mini Kit for up to 200 μg of protein

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is also highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl₂, NaCl, KCl, CaCl₂, LiCl and CsCl.  The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is also designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.  Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli.  Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications.  This kit efficiently reduces endotoxin levels to ≤ 0.1 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix.  The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes

Performance Data

Figure 1. Endotoxin-Free Proteins. Endotoxins were removed from 1 mgprotein samples in triplicate using the ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit. On average, the endotoxin levels in the samples were reduced to 0.07 EU/mg of protein.

Figure 2. High Protein Recovery. An average protein recovery of greater than 95% was achieved when endotoxins were removed from 0.5 mg of protein in triplicate.There is very little loss of protein associated with using the ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit.