CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows
A Fusion of Proteins A and G to Micrococcal Nuclease
Product Description
Recombinantly produced in E. coli, CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets & Release Using Nuclease (CUT&RUN). CUTANA™ pAG-MNase contains a C-terminal 6xHis epitope tag.
Add EpiCyphers Library Prep Kit for a streamlined CUT&RUN workflow.
Formulation
CUTANA™ pAG-MNase is provided as a 20X stock in 10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50% glycerol.
Storage and Stability
Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
References
- Skene PJ, Henikoff JG & Henikoff S (2018) Targeted in situ genome-wide profiling with high efficiency for low cell numbers. Nature Protocols
- Skene PJ, Henikoff S (2017) An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. eLife
- Schmid M, Durussel T & Laemmli UK (2004) ChIC and ChEC; genomic mapping of chromatin proteins. Molecular Cell
- Catalog Number
15-1116-EPC - Supplier
EpiCypher - Size
- Shipping
Blue Ice
Price
1.516,00 €
