CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows
A Fusion of Proteins A and G to Micrococcal Nuclease
Product Description
Recombinantly produced in E. coli, CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets & Release Using Nuclease (CUT&RUN). CUTANA™ pAG-MNase contains a C-terminal 6xHis epitope tag.
Add EpiCyphers Library Prep Kit for a streamlined CUT&RUN workflow.
Formulation
CUTANA™ pAG-MNase is provided as a 20X stock in 10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50% glycerol.
Storage and Stability
Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
Product Citations
- James A Oo, Timothy Warwick, Katalin Pálfi, Frederike Lam, Francois McNicoll, Cristian Prieto-Garcia, Stefan Günther, Can Cao, Yinuo Zhou, Alexey A Gavrilov, Sergey V Razin, Alfredo Cabrera-Orefice, Ilka Wittig, Soni Savai Pullamsetti, Leo Kurian, Ralf Gilsbach, Marcel H Schulz, Ivan Dikic, Michaela Müller-McNicoll, Ralf P Brandes, Matthias S Leisegang (2025) Long non-coding RNAs direct the SWI/SNF complex to cell type-specific enhancers. Nature communications
- Janeva Ana, Penfold Christopher A., Llorente-Armijo Sara, Li Huiwen, Zikmund Tomas, Stock Marco, Jullien Jerome, Straub Tobias, Forne Ignasi, Imhof Axel, Vaquerizas Juan M., Gurdon John B., Hörmanseder Eva (2025) ‘Digital Reprogramming’ Decodes Epigenetic Barriers of Cell Fate Changes bioRxiv
- Qingfei Zheng, Benjamin H. Weekley, David A. Vinson, Shuai Zhao, Ryan M. Bastle, Robert E. Thompson, Stephanie Stransky, Aarthi Ramakrishnan, Ashley M. Cunningham, Sohini Dutta, Jennifer C. Chan, Giuseppina Di Salvo, Min Chen, Nan Zhang, Jinghua Wu, Sasha L. Fulton, Lingchun Kong, Haifeng Wang, Baichao Zhang, Lauren Vostal, Akhil Upad, Lauren Dierdorff, Li Shen, Henrik Molina, Simone Sidoli, Tom W. Muir, Haitao Li, Yael David, Ian Maze (2025) Bidirectional histone monoaminylation dynamics regulate neural rhythmicity Nature
- Yaqiong Liu, Xianzhong Lau, Prabhakaran Munusamy, Carlos M Abascal Sherwell Sanchez, Daniel Snell, Mahesh Sangrithi (2025) Single-cell RNA-seq identifies protracted mouse germline X chromosome reactivation dynamics directed by a PRC2-dependent mechanism. Developmental cell
- Zhiyang Li, Zhuangzhuang Feng, Mengzhan Chen, Xinxiu Shi, Bijia Cui, Yujie Sun, Heng Zhang, Yinan Li, Caihong Chen, Yiqian Feng, Jingxia Han, Xuewu Xing, Huijuan Liu, Tao Sun (2025) Rbfox3 Promotes Transformation of MDSC-Like Tumor Cells to Shape Immunosuppressive Microenvironment. Advanced science (Weinheim, Baden-Wurttemberg, Germany)
References
- Skene PJ, Henikoff JG & Henikoff S (2018) Targeted in situ genome-wide profiling with high efficiency for low cell numbers. Nature Protocols
- Skene PJ, Henikoff S (2017) An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. eLife
- Schmid M, Durussel T & Laemmli UK (2004) ChIC and ChEC; genomic mapping of chromatin proteins. Molecular Cell
- Catalog Number
15-1016-EPC - Supplier
EpiCypher - Size
- Shipping
Blue Ice
Price
434,00 €
