Reporter Gene Assays
Reporter Gene Assays
Gene Expression and Cell Signaling Studies
The development of reporter gene assays has greatly facilitated gene expression and cell signaling studies. Reporters like Firefly Luciferase (FLuc), Renilla Luciferase (RLuc), secreted Gaussia Luciferase (GLuc), and Secreted Alkaline Phosphatase (SEAP) can be readily assayed subsequent to transfection and allow users a choice of signal that can be tailored to the required sensitivity, the available detection system, the application as well as the cellular system employed.
Luciferase Assays
Due to their bright signals and nearly non-existent background luminescence, luciferases are widely-used reporters of biological activity,
Firefly Luciferase Assays
The Luc-Pair Firefly Luciferase HS Assay Kit is the perfect choice for high-sensitivity detection of Firefly luciferase activity. It works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.) using microplate or single-tube luminometers, and is compatible with many other Firefly luciferase vectors.
The Luc-Pair Firefly Luciferase HT Assay Kit is a convenient system for high-throughput detection of Firefly luciferase (FLuc) activity in 96- or 384-well plates. The assay reagents can be added directly to cells in growth medium without washing or preconditioning, and are optimized for use with many different media containing serum (e.g. DMEM, RPMI1640, etc.).
Renilla Luciferase Assays
The Luc-Pair Renilla Luciferase HS Assay Kit is the perfect choice for high-sensitivity detection of Renilla luciferase activity. It works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.) using microplate or single-tube luminometers, and is compatible with many Renilla luciferase vectors.
The Luc-Pair Renilla Luciferase HT Assay Kit is a convenient system for high-throughput detection of Renilla luciferase activity in 96- or 384-well plates. The assay reagents can be added directly to cells in growth medium without washing or preconditioning, and are optimized for use with many different media containing serum (e.g. DMEM, RPMI1640, etc.)
Dual-Reporter Assays - Firefly Luciferase & Renilla Luciferase
The Luc-Pair Duo-Luciferase Assay Kit 2.0 provides a flexible system in a convenient 96-well plate format for measuring Firefly and Renilla luciferases sequentially. It works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.) using a microplate luminometer, and is compatible with many FLuc and RLuc vectors. Due to its enhanced stability, the Luc-Pair Duo-Luciferase Assay Kit 2.0 is the ideal solution for measuring e.g. the inhibitory effect of a miRNA on a target sequence.
The Luc-Pair Duo-Luciferase HS Assay Kit is designed for high-sensitivity applications.
The Luc-Pair Duo-Luciferase HT Assay Kit is a convenient system for high-throughput detection of Firefly luciferase (FLuc) and Renilla luciferase (Rluc) activities in 96- or 384-well plates. The assay reagents can be added directly to cells in growth medium without washing or preconditioning, and are optimized for use with many different media containing 0–10% serum (e.g. DMEM, RPMI1640, etc.) Firefly luciferase luminescence is produced by one reagent, while a second reagent simultaneously quenches the Firefly luciferase and produces Renilla luciferase luminescence.
Dual-Reporter Assays - Gaussia Luciferase & Secreted Alkaline Phosphatase
The Secrete-Pair Dual Luminescence Assay Kit is designed to analyze the activities of Gaussia Luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) in a dual-reporter system. Both GLuc and SEAP are secreted reporter proteins, permitting detection without cell lysis. The assay measures dual reporter signals and allows transfection normalization. A single GLuc reporter assay is also available.