Luc-Pair Luciferase Assay Kit 2.0

Product Description

• Enhanced stability
• Greater flexibility
• High sensitivity in a large dynamic range
• Great for any mammalian cell line
• Low background luminescence in the system
• Better reproducibility due to a longer lasting signal
• Simple to use and budget friendly
• Optimized for high-throughput screening

The Luc-Pair™ Duo-Luciferase Assay Kit 2.0 has been improved upon from the Luc-Pair™ Duo-Luciferase Assay Kit. With enhanced stability, it provides a more flexible system in the same convenient 96-well plate format as before for measuring firefly and Renilla luciferases sequentially. Due to its enhanced stability, the Luc-Pair™ Duo- Luciferase Assay Kit 2.0 is the ideal solution for measuring the inhibitory effect of a miRNA on a target sequence.

Applications and Advantages

• High stability - Luciferase activity remains greater than 80% for 30 minutes after addition of the enzymatic substrates
• Easy to use - less hands-on time and quick results
• Suitable for micro-plate format - save time and money
• Ideal for studying the interaction of precursor miRNA clones with miRNA 3' UTR target sequence clones

Figure 1. Stability of firefly luciferase and Renilla luciferase signals using GeneCopoeia (GCI) Luc-Pair Duo-Luciferase Assay Kit 2.0. HEK 293 cells were transfected with GeneCopoeia pEZX-MT06 miRNA reporter vector for 48 hours. A.) The  Luc and RLuc activity was measured as described in the procedure. B.) The Competitor’s dual-luciferase assay kit was used in comparison.

Figure 2. Linear relationship between emitted light and amount of luciferase expression vector transfected in HEK293. HEK 293 cells were transfected with GeneCopoeia pEZX-MT06 miRNA reporter vector for 48 hours. The FLuc (A) and RLuc (B) activities were measured in lysates from different numbers of cells after incubation for 3 min as described in the procedure.