Luc-Pair™ Renilla Luciferase HT Assay Kit
| Specifications | |
|---|---|
| Product Category: | Renilla Luciferase Assays |
Product Description
- Enhanced stability with a half-life of approximately one hour (Figure 1). Ideal for high-throughput assays
- Convenience - lyse cells in culture medium and measure luciferase activity simultaneously
- Flexibility - works well in many culture media, even with serum present (Figure 2)
- Versatility - designed for assays with many different eukaryotic (vertebrates, lower invertebrates) cells using micro-plates or single-tube luminescence reader
- Low background - very limited background luminescence, no subtraction is required from readings
- Reproducibility - designed to yield reliable, linear results for a concentration range over several orders of magnitude
The Luc-Pair™ Renilla Luciferase HT Assay Kit is a convenient system for high-throughput detection of Renilla luciferase (RLuc) activity in 96- or 384-well plates. The Luc-Pair™ Renilla Luciferase HT Assay reagents can be added directly to cells in growth medium without washing or preconditioning, and are optimized for use with many different media containing serum (e.g. DMEM, RPMI1640, etc.)
The Luc-Pair™ Renilla Luciferase HT Assay Kit works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.) and is compatible with many Rluc vectors from GeneCopoeia, Promega and other sources.
Figure 1. Stability of Renilla luciferase signal. The Luc-Pair™ Renilla Luciferase HT Assay Kit provides excellent RLuc signal stability, with a half life of approximately 1 hour. HEK 293 cells were transfected with Promega pGL4.75 reporter vector for 48 hours. Rluc activity was measured at various time points using the Luc-Pair RLuc HT Assay Kit as described in the procedure.
The Luc-Pair™ Renilla Luciferase HT Assay Kit allows excellent signal strength and stability in many different types of culture media, even with serum present (Figure 2).
Figure 2. Renilla luciferase activities in various media. HEK 293 cells were transfected with Promega pGL4.75 reporter vector for 48 hours. 5×10^4 transfected cells were suspended in DMEM without serum or the following types of media containing 10% serum: DMEM, RPMI1640, EMEM, IMDM, McCoy’s5A, F-12K, MEBM, ACL-4, L15, Cho-S-SFMII, NCTC109. Renilla luciferase activities were measured at various time points using the Luc-Pair RLuc HT Assay Kit as described in the procedure.
