Small RNA-seq library prep kits for microRNA, piRNA, and other short fragments
When you need transcriptome-wide microRNA, piRNA, tRNA fragment, snRNA, or degradation fragment quantification, small RNA-seq library prep handles the short fragments (15 to 200 nt) that mRNA-seq misses. Specialised adapter ligation, gel-based size selection, and bias-mitigation chemistry differ from standard library prep.
Pick by chemistry and input.
- Adapter chemistry. Older T4-RNA-ligase methods introduce sequence-bias. Newer randomized-adapter or template-switching chemistry reduces bias.
- Input. Low-input kits for biofluid samples accept 10 to 100 ng total RNA. Standard kits need micrograms.
- Size-selection. Gel-based for cleaner small-RNA capture. Automated bead-based faster but may bias size distribution.
- Multiplex capacity. Match to your sequencing pool budget.
- Caveat. For circulating miRNA biomarker work bias-reduced library prep is essential; standard kits can produce dramatically different miRNA-count rankings on the same input.
RealSeq Biosciences and TamiRNA focus on bias-reduced small-RNA library prep.
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