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Next Generation Sequencing Kits

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NGS library prep kits and sequencing reagents for every workflow

Get matched chemistry from sample to sequencing-ready library, for research or clinical next generation sequencing.

The eight sub-categories below cover whole-genome sequencing, RNA-seq, targeted gene panels and epigenomic profiling. They also cover 3D genome organisation, CRISPR screen readout, small RNA profiling, immune repertoire sequencing, and cloud-based variant analysis. They are organised by application type, so you can go straight to the chemistry you need. Most kits are formatted for Illumina short-read instruments, with long-read options where noted. Custom panels and service options are available when off-the-shelf kits do not cover your target regions or organisms.

  1. Application type first. For whole-genome or low-plex DNA sequencing, go to DNA Library Preparation. For epigenomics (CUT&RUN, CUT&Tag, ChIP-seq, ATAC-seq, bisulfite-seq), go to Epigenomic Profiling. For 3D genome or structural variant detection, go to Chromosomal Structure (Hi-C). For CRISPR screen readout, go to Genetic Screens and Downstream NGS. For targeted gene panels go to Targeted Sequencing, for immune repertoire sequencing go to Immune Receptor Profiling. For RNA and small RNA profiling, go to RNA Library Preparation.
  2. Sample type. For fresh or frozen cells and tissue, standard kits apply throughout. For FFPE tissue, get in touch to find the right solution. For low-input or single cells, use high-sensitivity kits made for limited starting material.
  3. Software and analysis. For cloud-based variant calling and clinical interpretation without local infrastructure, go to Bioinformatics.
  4. Custom or quote-based products. Custom capture panels, Hi-C modules, CRISPR guide libraries, small RNA suppression panels, and bioinformatics subscriptions are all quote-based. Contact BioCat to start a project.

Applications

Whole-genome and exome sequencing

Transposase-based DNA library preparation reagents turn genomic DNA into fragmented, adapter-ligated, indexed libraries for short-read or long-read sequencing. You can pair them with Hyb Capture Kits for Exome or targeted Sequencing.

Epigenomic profiling

ChIP-seq, ATAC-seq, CUT&RUN, and bisulfite sequencing map histone modifications, open chromatin, transcription factor binding, and DNA methylation across the genome. You can use them in cell lines, primary tissue, and clinical samples.

3D genome organisation

Hi-C and Capture Hi-C reveal topologically associating domains (TADs), compartment boundaries, and enhancer-promoter loops. These structures help regulate gene expression in development and disease.

Functional Genetic Screens

Pooled CRISPR-Cas9 loss-of-function and gain-of-function screens use lentiviral sgRNA libraries with NGS readout. This helps you find essential genes and drug targets in cancer, immunology, and infectious disease models.

Transcriptome profiling

Bulk and single-cell RNA-seq measure gene expression, detect splice variants, and resolve cell-type differences. You can use them in tissues, organoids, and patient samples.

Targeted sequencing

Hybrid capture panels and immune repertoire kits focus sequencing reads on defined genomic regions, rare variants, or T cell and B cell receptor V-D-J junctions. This makes them useful for translational and clinical research. 

Frequently Asked Questions

Which sequencing platforms do these kits support?
 Most kits are formatted for Illumina short-read instruments, with long-read options for PacBio where noted. Check the sub-category for platform compatibility before you order. 
How do I choose the right NGS kit?

 Start from the application. Pick DNA library prep for whole-genome or targeted DNA work, epigenomic profiling for chromatin and methylation, or RNA library prep for expression and small RNA. The sub-categories above route you to the matched chemistry. 

Can you supply custom or quote-based products?
 Yes. Custom capture panels, Hi-C modules, CRISPR guide libraries, and bioinformatics subscriptions are quote-based, for when off-the-shelf kits do not cover your target regions or organisms. 
What sample types can these kits handle?

Standard kits work with fresh or frozen cells and tissue. FFPE-compatible and low-input formats handle degraded or limited material, down to single cells.

 

Product catalogs

Not sure which catalog to start with? We will help you find the right products.

Tell us what you are looking for — antibodies, kits, proteins, or supplies — and our team will point you to the best catalog filters, suppliers, and product matches for your workflow.

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