Genotyping qPCR Mixes

qPCR master mixes for SNP genotyping and allelic discrimination assays

qPCR master mixes optimised for SNP genotyping and allelic discrimination work with dual-probe assays (different fluorophore per allele) to give simultaneous detection and discrimination in one well. Used in genetic screening, SNP-based diagnostics, and pharmacogenomic profiling.

Pick by chemistry and sample input.

1. Chemistry. TaqMan dual-probe is standard. Each allele gets its own fluorophore-quencher pair.
2. LightCycler hybridisation probes. Alternative chemistry for melt-curve-based genotyping.
3. Inhibitor tolerance. For crude lysate input directly from saliva, blood, or tissue choose mixes that accept crude lysate. Saves a sample-prep step in high-throughput genotyping.
4. Multiplex. Genotyping mixes are typically duplex (two alleles). For larger panels choose multiplex-validated chemistry.
5. Throughput. For 384-well or 1536-well formats verify the mix is validated for the volume.

Meridian Life Science is the primary supplier in this catalogue.