cDNA Synthesis for RT-qPCR

cDNA synthesis kits optimised for high-throughput, short-amplicon RT-qPCR workflows

cDNA synthesis kits specifically optimised for downstream RT-qPCR rather than other applications. These kits are priced and packaged for high-throughput single-reaction use, with priming chemistry optimised for short-amplicon qPCR rather than full-length cDNA.

Pick by priming and input.

1. Priming strategy. Random hexamer or oligo-dT or both. Oligo-dT for poly-A mRNA only. Random hexamer for unbiased coverage including non-poly-A targets.
2. Input range. For biofluid or low-input applications choose nanogram-compatible kits.
3. RT enzyme. Match enzyme to your downstream qPCR (sensitivity, fidelity, GC tolerance).
4. qPCR compatibility. Verify the resulting cDNA is compatible with your master mix.
5. miRNA. For miRNA detection separate stem-loop or polyA-tailed RT chemistry is required; standard cDNA synthesis misses mature miRNAs.

SBI System Biosciences and Meridian (Bioline) lead this catalogue.