TaqMan Probes and Primers

BHQ Dual-Labeled FRET Probes

Dual-labeled BHQ™ Probes are traditional, linear, FRET probes (TaqMan Probes) incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences.

STANDARDISE YOUR ASSAY - Dual-labeled BHQ probes are traditional probes that contain a 5’ fluorophore and a 3’ Black Hole Quencher dye covalently bound to an oligo. Choose from a broad spectrum of proprietary and common market fluorophores and their corresponding Black Hole Quencher dye to obtain high quality qPCR data.