qPCR Probe & Primer Synthesis Service

Custom DNA Probes for qPCR

Double-quenched BHQnova Probes

The most difficult qPCR applications demand double-quenched probes for optimum performance. BHQnova™ probes have improved quenching efficiency compared to traditional end-labeled probes, while also improving signal release upon amplification. BHQnova is most advantageous in longer probe designs, typically those over 25 bases, to boost the signal-to-noise ratio by overcoming the upper limit on sequence length.

BHQplus Probes

An advanced technology for qPCR and SNP genotyping, the BHQplus™ Probe is a compact oligo fortified with our signature duplex stabilising chemistry to elevate the melting temperature and enhance target specificity. BHQplus Probes are the optimal choice for marker-assisted selection in the agricultural industry, and to research the polymorphisms that may influence human health.

TaqMan Probes and Primers

A large selection of fluorophore-quencher combinations for dual-labeled probes is available. After synthesis the high quality BHQ Dual-Labeled FRET Probes (TaqMan Probes) are purified by HPLC to ensure every order meets purity requirements. Every oligo receives rigorous QC by electrospray mass spectroscopy before releasing for shipment.

Scorpions Primers

Scorpions™ Primers combine the probe and primer into one oligo to accomplish a uni-molecular signaling mechanism. Enzymatic hydrolysis of the probe during PCR cycling is not required for signal release.

Molecular Beacons

Molecular Beacons have short complementary sequences that fold into a stem-loop structure. This hairpin conformation positions the fluorophore and quencher very close together in space, for remarkably efficient quenching. LGC, Biosearch Technologies currently offers Molecular Beacons labeled with either a Black Hole Quencher™ or a DABCYL dye. These quenchers are paired with a variety of fluorescent reporters.