Gibson Assembly HiFi Kit

Gibson Assembly® Cloning Technology for assembly of up to 5 DNA fragments

Product Description

Complex assemblies of > 90% cloning efficiencies in 1 hour with less than 5 minutes hands-on time
Clone without the need for restriction enzyme sites
Optimal for fragment sizes ranging from 500bp to 32kb
Utilize any vector using simple design strategies - avoid subcloning
Multiple Inserts - Assembles up to 5 inserts at one time
Create constructs up to 100kb in length in multi stage assemblies
Enhanced cloning specificity of PCR products
Easily adapted for multiple DNA manipulations, ss oligonucleotides and assembling repetitive sequences
Gibson Assembly method has been cited by more than 1,200 scientific publications since 2009

Gibson Assembly HiFi Technology utilizes a special mix of enzymes optimized to assemble the DNA quickly and efficiently. Unlike other approaches, SGI-DNA’s Gibson Assembly process utilizes Phusion^® High-Fidelity DNA polymerase for 3' end extension. This results in higher numbers of seamless error free clones ready for multiple downstream applications.

Applications

Clone multiple inserts into a vector without relying on the availability of restriction sites
Large Fragment Assemblies - Assemble gene clusters and genome-size fragments
Site-directed mutagenesis to make simultaneous changes in a single reaction

How It Works

Gibson Assembly method employs an innovative blend of enzymes in a single tube reaction in a 1 hour isothermal reaction. The process begins by designing dsDNA fragments with 20 – 40bp overlapping ends. The DNA fragments are mixed with the Gibson Assembly mix for assembly.

5' chew back
DNA fragments anneal
Phusion® DNA polymerase extends 3’ ends
Any nicks are sealed

The resulting construct is a double stranded, fully sealed, and error free molecule that can be used immediately for numerous molecular biology applications such as direct transformation, PCR, RCA, and other molecular biology applications.

Figure 1: The Gibson Assembly HiFi kit workflow

For complex constructs

For more complex constructs with up to 15 fragments the Gibson Assembly Ultra Kit is recommended.

Primer Design Tool

To assist you in designing primers for the assembly of DNA fragments the Gibson Assembly Primer Design Tool has been developed.

Commercial Use Rights

All Gibson Assembly^® products purchased from BioCat as Telesis Bio (Codex DNA) authorized distributor include full commercial use rights. Please contact us in case further details are needed.