Cold Fusion Cloning from System Biosciences (SBI) is a quick and easy way to place any insert - or multiple inserts simultaneously - at any site into any linearized vector. Highly efficient, with more than 90% positive cloning rate, with a restriction enzyme, phosphatase and ligase free system.
To accommodate diverse tagging needs (different tag types and/or different locations), our partner OriGene devised the PrecisionShuttle System to allow easy subcloning of an ORF from one tagged vector to another. A large panel of destination vectors is available so that you can express the ORF with different tags, with tags at different ends of the protein, or as the native, untagged protein depending on the application.