Stellaris® FISH Probes, Yeast RPO21 with CAL Fluor® Red 590

Stellaris® FISH Probes, Yeast RPO21 with CAL Fluor® Red 590 consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions.

Designed to detect RPO21 transcripts in specimens using fluorescence in situ hybridization (FISH).

Design Criteria

Product was designed against DNA-directed RNA polymerase II core subunit RPO21 a.k.a. RPB1; RPB220; SUA8 (NCBI gene ID:853395) and nucleotides 1-5202 of NM_001180200.1. This is an exclusive probe set.

The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Fluorescence Parameters

• Absorption Maximum (Lambda Max): 495 nm
• Fluorescence Maximum: 520 nm

Product Usage

Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles.

For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.

For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.

Protocols

Detailed protocols by sample type can be found here.