Stellaris® FISH Probes, Mouse Wnt5a with CAL Fluor® Red 610

Stellaris® FISH Probes, Mouse Wnt5a with CAL Fluor® Red 610 consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions.

Designed to detect Wnt5a transcripts in specimens using fluorescence in situ hybridization (FISH).

Design Criteria

Product is intended to target wingless-type MMTV integration site family, member 5A Wnt5a a.k.a. 8030457G12Rik, Wnt-5a (NCBI gene ID:22418) of NM_009524.3,XM_006518924.2,XM_006518926.2,NM_001256224.1,XM_006518923.2.

Fluorescence Parameters

• Absorption Maximum (Lambda Max): 548 nm
• Fluorescence Maximum: 566 nm

Product Usage

Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles.

For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.

For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.

Protocols

Detailed protocols by sample type can be found here.