Stellaris® FISH Probes, Human TGFB2 with CAL Fluor® Red 635

Stellaris® FISH Probes, Human TGFB2 with CAL Fluor® Red 635 consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions.

Designed to detect TGFB2 transcripts in specimens using fluorescence in situ hybridization (FISH).

Design Criteria

Product was designed against transforming growth factor beta 2 TGFB2 a.k.a. LDS4; TGF-beta2 (NCBI gene ID:7042) and nucleotides 1-2923 of NM_003238.3. This is an inclusive probe set design to detect the following variants: NM_003238.3, NM_001135599.2

The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Fluorescence Parameters

• Absorption Maximum (Lambda Max): 618 nm
• Fluorescence Maximum: 637 nm

Product Usage

Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles.

For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.

For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.

Protocols

Detailed protocols by sample type can be found here.