Stellaris® FISH Probes, Human SAT1 with CAL Fluor® Red 590 Dye

Stellaris® FISH Probes, Human SAT1 with CAL Fluor® Red 590 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions.

Designed to detect SAT1 transcripts in specimens using fluorescence in situ hybridization (FISH).

Design Criteria

Product was designed against Spermidine / spermine N1-acetyltransferase 1 SAT1 a.k.a. SAT; DC21; KFSD; SSAT; KFSDX; SSAT-1 (NCBI gene ID:6303) and nucleotides 1-396, 397-722 of NM_002970.2. This is an inclusive probe set designed to also detect the following variant: NR_027783.

The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Fluorescence Parameters

• Absorption Maximum (Lambda Max): 540 nm
• Fluorescence Maximum: 558 nm

Product Usage

Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles.

For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.

For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.

Protocols

Detailed protocols by sample type can be found here.