Stellaris® FISH Probes, Human NANOG | -P1 | -P8 with Quasar® 670 Dye

Stellaris® FISH Probes, Human NANOG | -P1 | -P8 with Quasar® 670 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions.

Designed to detect NANOG | -P1 | -P8 transcripts in specimens using fluorescence in situ hybridization (FISH).

Design Criteria

Product was designed against Nanog homeobox | Nanog homeobox pseudogene 1 | Nanog homeobox pseudogene 8 NANOG | -P1 | -P8 a.k.a. N/A | NANOG2 | PN8, NANOG, NANOGP1 (NCBI gene ID:79923 , 404635 , 388112) and nucleotides 409-725, 769-1361, 1665-2042 of NM_024865.3 . This is an inclusive probe set designed to detect the following variants: NANOG: NM_024865.3, NM_001297698, AB093576, XM_011520850.1, XM_011520851.1, XM_011520852.1 NANOGP1: AK097770, AY455283 NANOGP8: AY455284

The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Fluorescence Parameters

• Absorption Maximum (Lambda Max): 569 nm
• Fluorescence Maximum: 591 nm

Product Usage

Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles.

For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.

For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.

Protocols

Detailed protocols by sample type can be found here.