Stellaris® FISH Probes, Human IL2RA with Quasar® 570

Stellaris® FISH Probes, Human IL2RA with Quasar® 570 consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions.

Designed to detect IL2RA transcripts in specimens using fluorescence in situ hybridization (FISH).

Design Criteria

Product was designed against interleukin 2 receptor, alpha IL2RA a.k.a. CD25, IDDM10, IL2R, TCGFR, p55 (NCBI gene ID:3559) and nucleotides 1-1567 of NM_000417.2. This is an inclusive probe set designed to also detect the following variants: NM_001308242.1, NM_001308243.1

The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Fluorescence Parameters

• Absorption Maximum (Lambda Max): 548 nm
• Fluorescence Maximum: 566 nm

Product Usage

Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles.

For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.

For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.

Protocols

Detailed protocols by sample type can be found here.