Stellaris® FISH Probes, Human CDK2 with FAM

Stellaris® FISH Probes, Human CDK2 with FAM consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions.

Designed to detect CDK2 transcripts in specimens using fluorescence in situ hybridization (FISH).

Design Criteria

Product was designed against cyclin-dependent kinase 2 CDK2 a.k.a. CDKN2; p33(CDK2) (NCBI gene ID:1017) and nucleotides 1-356, 357-648, 649-2121 of NM_001290230.1 . This is an inclusive probe set design to detect the following variants: NM_052827.3, NM_001798.4, NM_001290230.1, and XM_011537732.1

The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Fluorescence Parameters

• Absorption Maximum (Lambda Max): 548 nm
• Fluorescence Maximum: 566 nm

Product Usage

Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles.

For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.

For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.

Protocols

Detailed protocols by sample type can be found here.