SeekOne™ Digital Droplet System

SeekOne Digital Droplet System enables automated single-cell partitioning, capture, and labeling for high-throughput single-cell and multi-omics workflows.

• Accelerate single-cell capture with an approximately 5-minute automated run
• Scale experiments across 1–8 samples with up to 8 independently operated channels
• Support diverse sample types including fresh, fresh frozen, and FFPE-derived cells or nuclei
• Minimize multiplets with a reported rate below 0.3% per 1,000 cells
• Integrate transcriptomic, spatial, and epigenetic workflows through compatible reagent kits

Product Details

Automated microfluidic partitioning for single-cell workflows

The SeekOne Digital Droplet System (M001A) uses microfluidic water-in-oil droplet technology to partition, capture, and label individual cells or nuclei. Precisely controlled compressed air coordinates the air supply, temperature control, compression, circuitry, and software modules to generate the droplet reaction system.

Rapid processing with flexible sample throughput

The system generates approximately 150,000 water-in-oil droplets in 3 minutes and completes single-cell capture in about 5 minutes. Eight channels support flexible operation of 1–8 samples per run, with current specifications reporting a throughput of 500–120,000 cells or nuclei per channel.

Broad compatibility with cell and nuclei inputs

Supported sample types include cells or nuclei derived from fresh tissue, fresh frozen tissue, and FFPE tissue, as well as PBMCs and protoplasts. The specified cell diameter range is 5–40 µm, allowing processing of different cell populations within the supported size range.

Low multiplet rate for single-cell partitioning

The system is specified for low multiplet rates of under 0.3% per 1,000 cells. Cell capture rates of up to 65% for the system’s single-cell workflow provide defined performance parameters for experimental planning.

Integrated temperature control for sample handling

The chip tray incorporates automatic temperature control to maintain stable cell conditions during processing. The operating environment is specified at +18°C to +28°C with relative humidity of ≤80%, while the instrument monitors case temperature and provides alerts outside the normal 4°C to 38°C range.

Compact instrument designed for laboratory benches

The main unit measures 260 mm × 215 mm × 230 mm and weighs 7.25 kg, with a 200 W power input and 80–240 V AC, 50/60 Hz power supply. A 7-inch capacitive touchscreen provides direct control of operating parameters, settings, event logs, firmware updates, and language configuration.

Compatible with multiple single-cell assay workflows

The system can be paired with compatible single-cell reagent kits for transcriptomic, spatial, and epigenetic applications. Listed companion workflows include 3' and 5' transcriptome sequencing, full-length RNA sequencing, immune profiling, spatial sequencing, scMethyl + RNA-seq, scATAC + RNA-seq, and FFPE single-cell RNA sequencing. The system is designated for research use only and is not for diagnostic use. 

Explore the SeekOne™ Digital Droplet System and related products within the SeekOne DD Series Portfolio. For detailed information about the complete range of SeekGene's Multi-Omics Single-Cell Kits please refer to the SeekOne DD Comparison Guide.

Questions?

Contact us to learn more about the SeekOne™ Digital Droplet System and its related single-cell reagent kits.