PinPoint-FC attP Placement Vector

Easily place the PinPoint attP site into a target genome with the PhiC31 Integrase Expression Plasmid and this PinPoint attP Placement Vector.

• Create isogenic cell lines
• Achieve high-efficiency integration with no insert size-limit
• Turn cell line construction into a high-throughput process

Leverage the efficient PhiC31 Integrase System to insert a PinPoint attP site into a target genome with the PinPoint-FC attP Placement Vector. In addition to the PinPoint attP site, the PinPoint-FC attP Placement Vector contains a neomycin resistance gene for selection of integrants and a PGK promoter. The PGK promoter is positioned so that, in the second step of PinPoint integration, the PGK promoter will drive a promoterless puromycin gene supplied by any of SBI’s PinPoint Donor Vectors.

With the PinPoint Targeted Integration System, you can ensure that only a single copy of your transgene is inserted into the genome.

Learn more about Targeted integration with the PinPoint System