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SKU: MET-5031-CB

NADP+/NADPH Assay Kit (Fluorometric)

Blue Ice Fluorometric

Specifications

Specification Details
Additional Information Our NADP+/NADPH Assay Kit detects NADP+ and NADPH in cell and tissue lysates. Total NADP+/NADPH can be detected or samples can be treated with an acid or base treatment to specifically detect NADP+ or NADPH. This assay uses an enzymatic cycling reaction that reduces NADP+ to NADPH, which then reacts with a fluorometric probe and is detected with a fluorescence plate reader at 530-570nm excitation and 590-600nm emission. NADP+/ NADPH levels in unknown samples are calculated based on the provided NADP+ standard curve.
Detection Method Fluorometric
Sample Type Compatible with cell or tissue lysates
Assay Category Coenzymes and Cofactors

Resources

PDF

Manual

Catalog Number
MET-5031-CB
Name
NADP+/NADPH Assay Kit (Fluorometric)
Price(100 assay(s))
Pack Size
100 assay(s)
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