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Metabolism & Oxidative Stress Assay Kits

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Metabolism and oxidative stress assay kits for energy, mitochondrial and redox biology

Studying energy metabolism and redox biology together often means switching between assay platforms for each readout. These kits bring both into your plate-based workflow. You measure mitochondrial function, antioxidant defence, and damage to biomolecules across a wide range of species and sample types.

The range spans energy and intermediary metabolism, oxidative and nitrosative stress, antioxidant defence, and damage to biomolecules. Most kits are simple, plate-based assays in colorimetric, fluorometric, or related formats. Choose the format that fits your samples and species.

These kits are intended for research use only and are not intended for diagnostic or therapeutic use.

Choosing the right metabolism and oxidative stress kit

  1. Analyte. Match the kit to the specific metabolic parameter or reactive species you need to measure.
  2. Sample and species. Choose an assay validated for your sample type and compartment, whether cells, tissues, lysates, supernatants, or biofluids, intracellular or extracellular. Confirm it is validated for your species.
  3. Detection format. Decide between live-cell kinetic assays and endpoint assays. Then choose a colorimetric, fluorometric, or related plate-based format that fits your instruments and throughput.
  4. Performance and sample background. Check that sensitivity and dynamic range cover your expected values. Check whether haemoglobin, lipids, phenol red, or strong natural colour or fluorescence in your sample could interfere with the signal.

Applications

Bioenergetics profiling

Measure ATP/ADP ratio, NAD+/NADH, and lactate/pyruvate to characterise glycolytic versus oxidative phosphorylation flux in your cancer lines and cardiomyocytes.

Redox biology

Superoxide detection, glutathione quantification, and antioxidant capacity assays map cellular redox balance, paired with fluorescent redox probes for live-cell imaging.

Metabolic disease models

Glucose uptake, insulin signalling, and fatty acid oxidation assays are core endpoints in diabetes and fatty liver research, often using your hepatocyte models.

Neurodegeneration research

Mitochondrial ROS production and lipid peroxidation, measured as 4-HNE or MDA, are established markers in Parkinson's and Alzheimer's models.

Drug toxicity screening

Mitochondrial membrane potential dyes and cytochrome c release assays detect liability in your compound panels, complementing broader cell-based assay panels.

Ageing and senescence research

ROS accumulation and mitochondrial membrane potential decline are senescence markers, tracked using dedicated ROS kits alongside SA-beta-galactosidase.

Frequently asked questions

What is the difference between a metabolism assay and an oxidative stress assay?

Metabolism assays measure the concentration or flux of specific metabolites. These include glucose, ATP, NADH, lactate, and amino acids, and they map energy pathway activity. Oxidative stress assays detect reactive oxygen species (ROS) and antioxidant enzyme activities, such as catalase and superoxide dismutase. They also detect lipid peroxidation products and oxidised protein or DNA damage markers. Scientists often measure both in the same experiment, because oxidative stress directly affects metabolic pathways.

 What sample types are compatible with metabolism and oxidative stress kits?

Most colorimetric and fluorometric kits accept cell lysates, tissue homogenates, cell culture media, blood plasma, and urine. Lysis buffer composition matters: detergent-based buffers can interfere with some enzyme activity assays. We recommend that you normalise protein concentration with a BCA or Bradford assay when you compare across sample types.
How do I measure mitochondrial versus cytoplasmic ROS separately?

Use mitochondria-targeted fluorescent probes to selectively detect mitochondrial superoxide. These probes build up in the mitochondrial matrix because of membrane potential. Measure cytoplasmic ROS with membrane-permeable dyes instead. These dyes are oxidised by a broad range of reactive species throughout the cell. To confirm mitochondrial compartmentalisation, use confocal colocalization with mitochondria-targeted fluorescent dyes.

Product catalogs

Not sure which catalog to start with? We will help you find the right products.

Tell us what you are looking for — antibodies, kits, proteins, or supplies — and our team will point you to the best catalog filters, suppliers, and product matches for your workflow.

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