SKU: E-BC-K046-M-500Assays-ELS
Lactate Dehydrogenase (LDH) Activity Assay Kit
Blue Ice
Microplate reader
Specifications
| Specification | Details |
|---|---|
| Assay Time | 45 min |
| Detection Range | 6-1000 U/L |
| Sensitivity | 6 U/L |
| Additional Information | Lactate dehydrogenase (LDH or LD) is an enzyme found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. LDH is expressed extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease such as heart failure. Lactate dehydrogenase is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein, encoded by the LDHA and LDHB genes, respectively. These two subunits can form five possible tetramers (isoenzymes): 4H, 4M, and the three mixed tetramers (3H1M, 2H2M, 1H3M). These five isoforms are enzymatically similar but show different tissue distribution: The major isoenzymes of skeletal muscle and liver, M4, has four muscle (M) subunits, while H4 is the main isoenzymes for heart muscle in most species, containing four heart (H) subunits. |
| Detection Method | Microplate reader |
| Sample Type | Serum,plasma,hydrothorax,tissue,cells |
| Assay Category | Energy Metabolism, Glycolysis |
Resources
PDF
Manual
PDF
Fact Sheet
Flyer Energy Metabolism Assay Kits
Catalog Number
E-BC-K046-M-500Assays-ELS
Name
Lactate Dehydrogenase (LDH) Activity Assay Kit
Price(500.00 assay(s))
Pack Size