SKU: T0814-GVO-ABM

Immortalized Preadipocyte Clonal Cell Line PGF3-3, Type 3

Mouse In Stock Dry Ice

Specifications

Specification	Details
Organism	Mouse
Additional Information	Collagenase digestion was used to isolate subcutaneous and perigonadal cells derived from 6-week old Immortomouse. Cells were grown at 33°C in preadipocyte growth media with 10 units/mL Interferon γ. Aria flow cytometry was used to sort 190 cells and place each one in 190 wells after two passages. Clonal cell lines were created and grown at 33°C with 10 units/ml interferon γ. The clonal cell lines were treated and tested for mycoplasma contamination for 2 weeks. Oil Red O staining was used after differentiation to test the adipogenic capacity of cell lines. Microarray analysis of gene expression is found at the Gene Expression Omnibus database (accession #GSE110531). Subpopulations of adipocytes have distinct cellular responses and metabolism and can be used to study behavioural and physiological differences in fat depots.

Specification

Details

Organism

Mouse

Additional Information

Collagenase digestion was used to isolate subcutaneous and perigonadal cells derived from 6-week old Immortomouse. Cells were grown at 33°C in preadipocyte growth media with 10 units/mL Interferon γ. Aria flow cytometry was used to sort 190 cells and place each one in 190 wells after two passages. Clonal cell lines were created and grown at 33°C with 10 units/ml interferon γ. The clonal cell lines were treated and tested for mycoplasma contamination for 2 weeks. Oil Red O staining was used after differentiation to test the adipogenic capacity of cell lines. Microarray analysis of gene expression is found at the Gene Expression Omnibus database (accession #GSE110531). Subpopulations of adipocytes have distinct cellular responses and metabolism and can be used to study behavioural and physiological differences in fat depots.

Catalog Number

T0814-GVO-ABM

Name

Immortalized Preadipocyte Clonal Cell Line PGF3-3, Type 3

Price(100 vial)

Please inquire

100 vial

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