SKU: EXEL-ULTRA-GroEL-1-SBI
ExoELISA-ULTRA Complete Kit (GroEL, for E.coli OMV Detection)
Description
- Sensitive - detect as little as 0.5 µg protein equivalent
- Fast - complete in less than 4-hours; no more overnight incubation
- Flexible - compatible with common OMV isolation methods (e.g. ExoBacteria™ OMV Isolation Kit, ultracentrifugation) from E.coli culture
- Quantitative - calibrated internal standards enable quantitation of OMVs carrying GroEL
- Sample-saving - requires significantly low sample amount, leaving more for other downstream applications
Completing the collection of our popular ExoELISA-ULTRA Kits, the ExoELISA-ULTRA GroEL Kit is brought to users as the first and only kit for easy quantitation and characterization of OMVs from E. coli, with supreme sensitivity of OMV detection - as low as 0.5 µg protein equivalent - and a total assay time of only 4 hours.
Configured for detection of E.coli OMVs, an OMV associated molecular chaperonin GroEL was selected as the bacterial OMV marker 1-4. The ExoELISA-ULTRA GroEL detection assay is based on an ultra-sensitive direct capture colorimetric ELISA that is compatible with OMVs isolated from E. coli culture by common OMV isolation methods. To enable quantitation of OMVs carrying GroEL in your target sample, the kit includes an internal standard calibrated to OMVs isolated with different OMV isolation methods that have been analyzed by nanoparticle tracking analysis (NTA). This assay is also predicted to work with OMVs derived from Shigella dysenteriae and Salmonella enterica.
Supporting Data
Exo-ELISA-ULTRA GroEL Standard Curve
Figure 1. ExoELISA-ULTRA GroEL standard curve shows robust linearity down to ~ 0.82 x 109 OMVs. OD 450 nm values of OMVs isolated with common OMV isolation methods (Ultracentrifuge and ExoBacteria™ OMV Isolation Kit fall well within the standard curve for the assay.
Overview of Exosome Quantitation Kits
