ExoELISA-ULTRA BamA Complete Kit (For Gram-neg. Bacterial OMV Detection)

• Sensitive - detect as little as 1 µg protein equivalent
• Fast - complete in less than 4-hours; no more overnight incubation
• Flexible - compatible with common OMV isolation methods (e.g. ExoBacteria™ OMV Isolation Kit, ultracentrifugation) from bacterial culture media
• Quantitative - calibrated internal standards enable quantitation of OMVs carrying BamA

Completing the collection of our popular ExoELISA-ULTRA Kits, the ExoELISA-ULTRA BamA Kit is brought to users as the first and only kit for easy quantitation and characterization of OMVs from Gram-negative bacteria, with supreme sensitivity of OMV detection - as low as 1 µg protein equivalent - and a total assay time of only 4 hours.

Bacterial outer membrane vesicles (OMVs) play crucial roles in pathogenesis and intercellular interactions, yet a universal set of OMV marker proteins remains elusive. BamA, an essential subunit of the β-barrel assembly machine (BAM), is a highly conserved outer membrane protein found in all Gram-negative bacterial species and consistently enriched in purified bacterial OMVs (1-4). To facilitate the detection of OMVs from Gram-negative bacteria, BamA was chosen as the OMV marker. A custom antibody targeting the conserved C-terminal peptide of BamA was developed to ensure reactivity across all Gram-negative bacterial species (5-6). The ExoELISA-ULTRA OMV detection assay employs an ultra-sensitive direct capture colorimetric ELISA method compatible with OMVs isolated by common methods. For quantifying BamA-carrying OMVs in your sample, the kit includes an internal standard calibrated to OMVs isolated with various methods, validated by nanoparticle tracking analysis (NTA).

Supporting Data

Exo-ELISA-ULTRA BamA Standard Curve

Figure 1. ExoELISA-ULTRA BamA standard curve shows robust linearity down to 3.61 x 109 OMVs. OD 450 nm values of OMVs isolated with common OMV isolation methods (Ultracentrifuge and ExoBacteria™ OMV Isolation Kit) fall well within the standard curve for the assay.

Overview of Exosome Quantitation Kits

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