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SKU: E-BC-K837-M-96T-ELS

Cell Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit

Blue Ice Microplate reader

Specifications

Specification Details
Assay Time 35 min
Detection Range 0.79-47.0 U/L
Sensitivity 0.79 U/L
Additional Information Mitochondrial complex IV, also known as cytochrome C oxidase, is one of the major enzymes in the mitochondrial respiratory chain. It oxidizes the reduced cytochrome C converted from mitochondrial complex III to oxidized cytochrome C and consumes oxygen to generate water. Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.
Detection Method Microplate reader
Sample Type cells
Assay Category Energy Metabolism, Oxidative Phosphorylation

Resources

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Manual

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Fact Sheet

Flyer Energy Metabolism Assay Kits

Catalog Number
E-BC-K837-M-96T-ELS
Name
Cell Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit
Price(96 tests)
Pack Size
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