SeqPure PCR Purification Kit, 5 ml
| Specifications | |
|---|---|
| Product Category: | DNA Clean-Up & Concentration |
Product Description
- Removes salts, dNTPs, primers, and enzymes in less than 30 minutes
- Excellent DNA recovery and DNA purity - superior to AmPure
- Highly efficient size selection of DNA > 100 bp
- Competitive pricing
- No centrifugation or vacuum filtration required
- No fancy buffers; just use ethanol and Tris-HCI/TE
- Easily automated
- Amenable to 96- and 384- well formats
- Scalable: Use the amount of beads you need
SeqPure™ PCR reagent is a Post PCR and Next Gen Library Prep Clean Up System based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons and to optimize DNA sequence quality.
The beads selectively bind DNA fragments 100 bp and larger in size. Simple washing steps will remove salts and other buffer constituents, primers, primer dimers, nucleotides, and enzymes prior to elution of the purified DNA > 100 bp with low salt buffer. The highly purified DNA is ready-to-use for downstream applications including Next Generation Sequencing.
1. SeqPure beads are added to the PCR product for DNA binding.
2. Contaminants and salts are washed off.
3. Purified DNA is eluted, ready to be used in subsequent applications.
Applications
- Sequencing (Sanger and Next Generation)
- Mutation detection and genotypying
- Fragment analysis
- Microarrays
- Restriction enzyme clean up
- Cloning
Excellent DNA Purity and Recovery
Comparison of SeqPure and AmPure in purifying 100 bp DNA ladder containing salts and protein: The purity was measured by spectrophotometry. Both the 260/280 and 260/230 absorbance ratios show that SeqPure is slightly more efficient at removing salts and proteins compared to AmPure.
Equal amounts of 100 bp ladder containing salts and proteins were purified with SeqPure beads and Ampure beads. Different buffers, TE and pre-warmed TE (for increasing the yield) were used for elution. Eluted DNA (8 µl each) were loaded and run on a 1% agarose gel. The gel shows excellent recovery of all fragments from 3000-100bp.
Highly efficient Size Selection
Ease of Automation
SeqPure avoids the use of spin-columns and other purification membranes in order to make the kit convenient to use manually or automate on the widest possible selection of laboratory automation. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol.
Purification with paramagnetic particles, rather than spin columns also reduces hydrodynamic shearing – improving the integrity of larger DNA fragments. The scalable nature of paramagnetic bead technology also makes the kit more flexible. If you are purifying small amounts of DNA, you can use fewer beads, and extract more samples per purification kit.
