Minute Yeast Mitochondria Isolation Kit

Product Description

Traditional protocols for yeast mitochondria isolation/extraction involve a range of centrifugation-based subcellular fractionation procedures. Typically the techniques include spheroplast preparation, glass-bead lysis using a homogenization instrument, differential centrifugation and several density gradient procedures using a variety of gradient media with ultracentrifugation. The procedures are very tedious and time consuming. Here we feature a simple and rapid protocol for yeast mitochondria enrichment. The procedure is gentle and instrument-free. Nativ mitochondrial proteins can be isolated from yeast in about one hour without ultracentrifugation.

This kit contains optimized detergent-free protein extraction buffers. The protein yield is in the range of 150-250 μg/sample. The materials provided are sufficient for 50 extractions.

SDS-PAGE (12%) of S.cerevisiae fractions.

Lane 1, Total cell lysate; Lane 2, Cytosolic fraction; Lane 3, Mitochondrial fraction