XStamp CD40L exosome CD40 receptor targeting lentivector

Product Description

Putting exosomes to work: Target exosomes to antigen-presenting cells

Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to antigen-presenting cells with XStamp-CD40L. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. The XStamp-CD40L Lentivector fuses CD40L sequences to the XStamp tag, ensuring that exosomes display the CD40L on their outer surface. Exosomes made from cells transfected or transduced with the XStamp-CD40L Lentivector will now be targeted to cells expressing CD40, primarily antigen-presenting cells.

XStamp Targeting in Action

Figure 1. XStamp with motilin specifically targets exosomes to GI cells. Motilin is a 22-amino acid polypeptide hormone that binds to the motilin receptor, which is exclusively expressed in the intestine. XStamp with motilin can be used to target exosomes to GI cells. METHODS: The XStamp-Motilin construct (Cat.# XSTP720PA-1-SBI) was transfected into HEK293 cells and after 48 hours, the exosomes were isoalted using ExoQuick-TC. The next day, the XStamp-Motilin exosomes were Exo-Fected with a Texas-Red-labeled siRNA to monitor exosome docking and delivery. The transfected XStamp-Motilin exosomes were then added to MDA-MB-231 Breast Cancer Cells (motilin receptor negative) and to HT-29 Colon Cancer Cells (motilin receptor positive). The cells were imaged after 24 hours for uptake of the Texas-Red-labeled siRNA delivery from the XStamped exosomes. The HT-29 colon cancer cells that are motilin receptor positive took up the XStamp-Motilin exosomes at a much higher rate than the MDA-MB-231 Breast Cancer (motilin receptor negative) cells.

SBI also offers a selection of pre-built targeting XStamp Lentivectors (see table Related Products below), as well as a clone-your-own XStamp Cloning and Expression Vector, Cat# XSTP710PA-1-SBI.