XStamp BHP1 exosome brain homing peptide targeting lentivector

Product Description

Putting exosomes to work: Target exosomes to brain cells

Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to brain cells with XStamp-BHP1. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. The XStamp-BHP1 Lentivector fuses the BHP1 peptide to the XStamp tag, ensuring that exosomes display BHP1 on their outer surface. Exosomes made from cells transfected or transduced with the XStamp-BHP1 Lentivector will now be targeted to brain cells.

XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.

Supporting Data

XStamp Targeting in Action

Figure 1. Both XStamp-NCAM and XStamp-BHP1 can target exosomes to neurons. METHODS: The XStamp-NCAM Lentivector (Cat.# XSTP721PA-1) and the XStamp-BHP1 Lentivector (Cat.# XSTP722PA-1), were transfected separately into mouse MSCs along with SBI’s XPack-GFP construct (Cat.# XPAK530PA-1), which packages GFP into the interior of exosomes to enable fluorescent tracking. Exosomes were isolated after 48 hours, and added to Neuro2a neuroblastoma cells in culture. The neurons were monitored for GFP delivery using phase and fluorescence microscopy after a 24-hour incubation.

SBI also offers a selection of pre-built targeting XStamp Lentivectors (see table Related Products below), as well as a clone-your-own XStamp Cloning and Expression Vector, Cat# XSTP710PA-1-SBI.