OxiSelect™ Cellular Antioxidant Assay Kit (Green Fluorescence)
| Specifications | |
|---|---|
| Research Area: | Oxidative Stress |
| Assay Category: | Reactive Oxygen Species (ROS) and Antioxidant Assays |
| Detection Method: | Fluorometric |
Product Information
The OxiSelect™ Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.
Product Citations
- Aquilino M, et al. (2018) Genotoxic effects of vinclozolin on the aquatic insect Chironomus riparius (Diptera, Chironomidae). Environ Pollut.
- Pagano, I. et al. (2016) Chemical profile and cellular antioxidant activity of artichoke by-products. Food Funct.
- Verbanac, D. et al. (2016) Synthesis and evaluation of antibacterial and antioxidant activity of novel 2-phenyl-quinoline analogs derivatized at position 4 with aromatically substituted 4 H-1, 2, 4-triazoles. J Enzyme Inhib Med Chem.
- Catalog Number
STA-349-CB - Supplier
Cell Biolabs - Size
- Shipping
RT
Price
618,00 €
