OxiSelect™ Superoxide Dismutase Activity Assay Kit
| Specifications | |
|---|---|
| Research Area: | Oxidative Stress |
| Assay Category: | Superoxide Dismutase Assay |
| Sample Type: | Suitable for use with plasma, serum, cells or tissues |
| Detection Method: | Colorimetric |
Product Description
The OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions. The included chromagen produces a water-soluble formazan dye upon reduction by superoxide anions. The activity of SOD is determined as the inhibition of chromagen reduction.
The Superoxide Dismutase Activity Assay is a fast and reliable kit for the measurement of SOD activity from cell lysate, plasma, serum, tissue homogenates. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, SOD standards and unknown protein samples.
Assay Principle
Superoxide anions (O2-) are generated by a Xanthine/Xanthine Oxidase (XOD) system, and then detected with a Chromagen Solution. However, in the presence of SOD, these superoxide anion concentrations are reduced, yielding less colorimetric signal.
Product Citations
- Chan, S.H. et al. (2017) SIRT1 inhibition causes oxidative stress and inflammation in patients with coronary artery disease. Redox Biol.
- Choi, Y. et al. (2017) Comparative toxicity of silver nanoparticles and silver ions to Escherichia coli. J Env.
- Lei Shen et al. (2017) C‑X‑C motif chemokine ligand 8 promotes endothelial cell homing via the Akt‑signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers. Experimental and Therapeutic Medicine.
- Mahmoud, S.S. et al. (2017) Protective effects of ivabradine (alone and combined with atenolol or enalapril) on experimentally-induced acute myocardial infarction in albino rats. Z.U.M.J.
- Oguchi T, et al. (2017) Cilostazol Suppresses Aβ-induced Neurotoxicity in SH-SY5Y Cells through Inhibition of Oxidative Stress and MAPK Signaling Pathway. Front Aging NeuroSci
- Catalog Number
STA-340-CB - Supplier
Cell Biolabs - Size
- Shipping
Dry Ice
