Minute Detergent-Free Total Protein Extraction Kit for Fresh Plant Tissues (Native Proteins obtained)

Product Description

Minute™ detergent-free plant protein extraction kit

Invent Biotechnologie's Minute™ detergent-free plant protein extraction kit is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract detergent-free total proteins from plant tissues. The protein extraction buffer does not contain any detergent and organic solvent. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 50 μl and as large as 500 μl. Detergent-free total proteins can be extracted from very small plant samples (leaves, seeds, soft stems etc.) in less than 8 min with high yield (1-6 mg/ml).

Comparison of plant protein profiles extracted by denaturing lysis buffer and detergent-free extraction buffer. Lane 1, Corn kernel; Lane 2, Broccoli leaves; Lane 3, Spinach leaves. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.