Saliva RNA Collection and Preservation Devices

Product Description

• Reliable and cost-effective
• Non-invasive, user-friendly RNA sample collection
• Sample collection and preservation in one convenient kit
• Preserved RNA is stable for up to 2 months at room temperature
• Compatible with Norgen's Total RNA Isolation Kit (see link below)
• High quality RNA is suitable for sensitive downstream applications including RT-PCR, RT-qPCR, RNA-seq and microarray

Norgen's Saliva RNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of RNA in saliva samples at ambient temperature. Each of the 50 Saliva RNA Collection and Preservation Devices consists of 3 components: (1) Saliva Collection Funnel and Collection Tube, (2) Collection Tube Cap, and (3) Norgen's Saliva RNA Preservative contained within a sealed squeezable ampoule. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. RNA can be isolated from the preserved saliva samples using Norgen's Total RNA Purification Kit (Cat# 17200-NB), which yields truly representative total RNA including miRNA without the use of phenol. Each of Norgen's Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The Saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for epidemiological and population studies.

Stability of RNA preserved in Norgen's Saliva RNA Preservative at room temperature for up to 2 months. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva RNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 2 months. Saliva DNA was subsequently isolated at day 0, day 4, day 6, day 16, 1 month and 2 months from 0.25 mL of the saliva/preservative sample using Norgen's Total RNA Purification Kit (Cat. 17200-NB). For visual analysis, 7.5 µL from 50 µL elution was mixed with RNA loading dye and was run on an 1x MOPS agarose gel. RNA integrity was maintained after the saliva samples are stored up to 2 months at ambient temperatures in Norgen's Saliva RNA Preservative.

Stability of three house keeping genes over a 2 month period. GAPDH, Human 18S rRNA and beta actin genes were monitored from saliva samples stored at ambient temperature with Norgen's Saliva RNA Preservative. RNA was isolated from 250 µL of preserved samples on day 0, day 4, day 6, day 10, day 16, day 30 and day 60 using Norgen's Total RNA Purification Kit (Cat. 17200-NB). Next, 4 µL of elution was used in 20 µL of the real-time PCR reaction (SYBR Green I) using CFX96 TouchTM Real-Time PCR Detection System (Bio-Rad). All of the genes were preserved and stable over 2 month period.