ExoCt™ SYBR® Green RT-qPCR Kit

Product Description

The ExoCt™ SYBR® Green RT-qPCR Kit includes both RT and qPCR reagents, which combines PCR technology and SYBR® Green to make fast and accurate quantification of exosome RNAs. The system includes the ExoCt™ First-Strand cDNA Synthesis Kit and the ExoCt™ SYBR® Green RT-qPCR Kit.

The GeneCopoeia's ExoCt™ RT-qPCR System allows you to analyze gene expression by real-time qPCR directly from exosomes without any RNA purification.

Workflow

Isolated exosomes are lysed by adding ExoCt™ Lysis Buffer, then heating at 37°C for 10 min and 75°C for 10 min. Exosome lysates are used directly for ExoCt™ RT reactions, and the cDNA products can be used for qPCR to detect miRNA, mRNA, and LncRNA, etc.