ExoCt™ First-Strand cDNA Synthesis Kit

Product Description

The ExoCt™ First-Strand cDNA Synthesis Kit contains ExoCt™ RT-for-All™ Buffer and ExoCt™PAP/RTase Mix, which were specifically developed for robust first-strand cDNA synthesis covering a variety of RNA templates, including miRNA, mRNA, LncRNA, etc., from exosome lysates in a single reaction.

The GeneCopoeia's ExoCt™ RT-qPCR System allows you to analyze gene expression by real-time qPCR directly from exosomes without any RNA purification.

Workflow

Isolated exosomes are lysed by adding ExoCt™ Lysis Buffer, then heating at 37°C for 10 min and 75°C for 10 min. Exosome lysates are used directly for ExoCt™ RT reactions, and the cDNA products can be used for qPCR to detect miRNA, mRNA, and LncRNA, etc.