BlazeTaq™ One-Step SYBR Green RT-qPCR Kit (with ROX)

Product Description

BlazeTaq™ One-Step SYBR® Green RT-qPCR kit uses a reverse transcriptase to convert RNA to cDNA, and an antibody-modified Taq DNA polymerase to avoid polymerase activity prior to thermal cycling. Upon heating to 95 ºC for 3 min, the antibody dissociates and full activity of the Taq polymerase is restored. Also, the optimized buffer system allows high amplification efficiency and specificity, as well as enhanced sensitivity of real time PCR reactions over a wide range of templates.

• One-step reaction to quantify the relative amount of RNA
• High sensitivity- accurate detection of target RNA from as little as 0.1 pg RNA extract
• High specificity, with minimal level of primer-dimer and non-specific product formation
• High amplification efficiency over wide GC-content range

Performance

Figure 1. Analysis of the level of GAPDH and Actin in RNA extract from HeLa cells.

A. Amplification curves of GAPDH and Actin respectively in HeLa cells RNA extract ranging from 100 ng to 0.1 pg; B. Amplification curves of GAPDH and Actin respectively in HeLa cells RNA extract ranging from 100 ng to 0.1 pg; C. Melting curves of the amplified fragments of GAPDH (red) and Actin (blue).

Figure 2. Comparison of the performance of BlazeTaq™ RT-qPCR kit with competitor's product.

A. Amplification curves of GAPDH in HeLa cells RNA extract ranging from 100 ng to 0.1 pg with BlazeTaq™ (red) and Competitor T's kit (blue); B. Amplification curves of Actin in HeLa cells RNA extract ranging from 100 ng to 0.1 pg with BlazeTaq™ (red) and Competitor T's kit (blue).

Figure 3. Comparison of the performance of BlazeTaq™ RT-qPCR kit with competitor's product.

A. Amplification curves of GAPDH in HeLa cells RNA extract ranging from 100 ng to 0.1 pg with BlazeTaq™ (red) and Competitor P's kit (blue); B. Melting curves of amplified fragments of GAPDH with BlazeTaq™ (red) and Competitor P kit (blue). The peak shows non-specific amplification from competitor P's kit.