1-Step dye-based RT-qPCR Systems enable detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining Reverse Transcriptase and Taq qPCR Master Mix in a single reaction well.
The SensiFAST SYBR® One-Step Kits use a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. SensiFAST One-Step Kits consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.
To find the optimal SensiFAST kit for your real-time PCR instrument and application, you can also use this Real-Time PCR Selection Chart.