EpiQuik Magbeads Quick RNA Isolation Kit

Product Description

• Fast procedure delivers high-quality total RNA in approximately 30 minutes
• Ready-to-use RNA for high performance in any downstream application
• Consistent RNA yield from a small amount of starting material

The EpiQuik™ Total RNA Isolation Fast Kit provides a fast, simple, and cost-effective method for the isolation of total RNA from mammalian cells and tissues. Detergents are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows protein/DNA to be removed and RNA to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted. The RNA purified with this kit is suitable for a variety of routine applications, including RT-PCR, cDNA synthesis, northern blotting, differential display, primer extension, and mRNA selection. The entire procedure can be completed within 25-40 minutes. The kit has the following features and advantages:

Principle & Procedure

This kit contains all the reagents required for successfully performing RNA isolation directly from cultured cells or tissues. After lysis, binding, and wash, RNA is easily recovered in quantities of up to 10 µg using specially designed columns. Total RNA is then ready to be used for a variety of downstream applications.

Starting Materials

The amount of starting materials can be up to 1 X10^6 mammalian cells, and 10 mg tissues. A total of 50 standard extractions can be performed with this kit.

Performance Data

Fig 1. The isolated RNA using the EpiQuik™ Magbeads Quick RNA Isolation Kit was spiked into FBS and re-isolated/purified with the kit to determine the recovery efficiency. The concentrations were and fluorescently quantified using a fluorescent method for RNA/ssDNA measurement.