EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)
Specifications | |
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Product Category: | Cell Fractionation |
Sample Type: | Mammalian Cells, Tissues |
Product Description
The EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free) is a complete set of optimized reagents to provide a simple and selective method for isolating nuclear proteins free of nucleic acids attached, to be used in a variety of applications in just 60 minutes. These applications may include Western blot, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nucleic acid-free nuclear proteins but not enzymatic activity. The protocol is fast and easy to use and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples. Some of the kit's highlighted features include the following:
- Pre-optimized and simple step-wise 1-hour protocol.
- Conveniently includes all essential reagents to carry out a nucleic acid-free nuclear extraction.
- Standardized procedure for reproducible results.
Principle & Procedure
The EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free) simply applies our proprietary nuclear protein isolation buffers to cells/tissues. After pre-lysis and lysis buffers are treated, the nucleic acid-free nuclear proteins are easily extracted for immediate use or storage under proper conditions.
Note: This item was formerly catalog no. OP-0002-2 (OP00022, OP-00022, OP0002-2)
Product Citations
- Nakazawa Y, Miyano M, Tsukamoto S, Kogai H, Yamamoto A, Iso K, Inoue S, Yamane Y, Yabe Y, Umihara H, Taguchi J, Akagi T, Yamaguchi A, Koga M, Toshimitsu K, Hirayama T, Mukai Y, Machinaga A (2024) Delivery of a BET protein degrader via a CEACAM6-targeted antibody-drug conjugate inhibits tumour growth in pancreatic cancer models. Nat Commun
- Pirola CJ, Salatino A, Fernández Gianotti T, Castaño GO, Garaycoechea M, Sookoian S (2024) Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease. EBioMedicine
- Possamai-Della T, Cararo JH, Aguiar-Geraldo JM, Peper-Nascimento J, Zugno AI, Fries GR, Quevedo J, Valvassori SS (2023) Prenatal Stress Induces Long-Term Behavioral Sex-Dependent Changes in Rats Offspring: the Role of the HPA Axis and Epigenetics. Mol Neurobiol
- Arora L, Patra D, Roy S, Nanda S, Singh N, Verma AK, Chakraborti A, Dasgupta S, Pal D (2022) Hypoxia-induced miR-210-3p expression in lung adenocarcinoma potentiates tumor development by regulating CCL2-mediated monocyte infiltration. Mol Oncol
- Barks AK, Beeson MM, Hallstrom TC, Georgieff MK, Tran PV (2022) Developmental iron deficiency dysregulates TET activity and DNA hydroxymethylation in the rat hippocampus and cerebellum. Dev Neurosci
- Catalog Number
OP-0022-100-EP - Supplier
EpigenTek - Size
- Shipping
Blue Ice