SOD (Superoxide Dismutase) Activity Assay/Inhibitor Screening Assay Kit (Colorimetric)
| Specifications | |
|---|---|
| Research Area: | Oxidative Stress |
| Assay Category: | Superoxide Dismutase Assay |
| Sample Type: | Cell extracts |
| Detection Method: | Colorimetric |
Product Description
- The fastest procedure available, which can be finished within 60 minutes.
- Very simple -- just one step.
- High throughput microplate format with consistent reaction conditions.
Determination of SOD activity is necessary in various research fields related to human diseases. There are several methods used for measuring SOD activity, yet most of these methods have some drawbacks in selectivity, rapidity, and convenience. The EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) addresses these problems by using a unique procedure to measure SOD activity.
Principle & Procedure
The EpiQuik™ Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) utilizes a dye that produces a water-soluble formazan upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by measuring superoxide anion-caused formation of water-soluble dye (color intensity). The higher the SOD activity, the less the formation of formazan and the lower the OD value.
Performance Data
Fig.1. Inhibition Curve Prepared Using SOD from Bovine Liver. The kit utilizes a water-soluble tetrazolium salt that produces a water-soluble formazan dye upon reduction with a superoxide anion (6). The rate of the reduction with O2- is linearly related to the xanthine oxidase (XO) activity and is inhibited by SOD. Therefore, the IC 50 (50% inhibition activity of SOD or SOD-like materials) can be determined by this colorimetric method.
Product Citations
- González-Domínguez Á, Visiedo F, Domínguez-Riscart J, Ruiz-Mateos B, Saez-Benito A, Lechuga-Sancho AM, Mateos RM (2021) Blunted Reducing Power Generation in Erythrocytes Contributes to Oxidative Stress in Prepubertal Obese Children with Insulin Resistance. Antioxidants (Basel)
- Calzetta L et al. (2018) N-Acetylcysteine protects human bronchi by modulating the release of neurokinin A in an ex vivo model of COPD exacerbation. Biomed Pharmacother 103:43108
- Qian Y et al. (2018) Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury. Cell Physiol Biochem 45(6):2268-2282
- Ashwini P. Aithal et al. (2017) Therapeutic Potential of Human Bone Marrow Derived Mesenchymal Stromal Cells in Combination with Silymarin against Carbon Tetrachloride Induced Liver Cirrhosis in Wistar Rats JKIMSU 6(4)
- Diao Z et al. (2017) Increased renal oxidative stress in salt-sensitive human GRK4γ486 V transgenic mice. Free Radic Biol Med
- Catalog Number
OP-0001-1-EP - Supplier
EpigenTek - Size
- Shipping
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