ZYCY10P3S2T E. coli Minicircle producer strain (competent, ready to transform)

Produce minicircles from SBI’s Parental Vectors with the ZYCY10P3S2T Minicircle Production Strain

Product Description

Optimized for minicircle production, the ZYCY10P3S2T Minicircle Production Strain comes as competent cells, so you can directly transform ligation reactions or plasmids. The competent cells are prepared and tested using a modification of the procedure developed by Kay, et al.¹, and are suitable for cloning and production of minicircle-based plasmids.

The ZYCY10P3S2T E.coli Minicircle Production Strain is derived from the BW27783 E. coli strain that stably expresses a set of inducible minicircle-assembly enzymes, PhiC31 integrase, and I-SceI homing endonuclease. This bacterial strain enables production and purification of minicircles in a time frame and quantity similar to that of routine plasmid DNA preparation.

Please note that other competent E. coli strains cannot be substituted for minicircle production as they do not have the appropriate genomic modifications to produce minicircles.

Arabinose-inducible System

The Minicircle Producer Strain harbors an arabinose-inducible system to express the PhiC31 integrase and the I-SceI endonuclease simultaneously. The ZYCY10P3S2T strain also contains a robust arabinose transporter LacY A177C gene. Adding arabinose to the media turns on expression of the PhiC31 integrase and endonuclease genes, resulting in separation of the Parental Minicircle Plasmid into the individual minicircle and parental plasmids (from the PhiC31 Integrase activity), and the degradation of the parental plasmid (from I-SceI endonuclease activity).

Related Products

Arabinose induction solution
Kanamycin solution
MC-Easy Minicircle DNA Production Kit

Please note

Parental minicircle plasmids and the ZYCY10P3S2T Producer Bacterial Strain are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made mcDNA [ready-to-transfect] only. For any other purposes, including the ability to buy the parental MC production system, commercial users should contact us at BioCat for additional details.

Minicircle Service

In cooperation with SBI BioCat also offers custom parental plasmid cloning and mcDNA production to both not-for-profit and commercial end users. Please contact us if you are interested in this service.

Citations and References

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