Arabinose induction solution (20%)

Product Description

Induce minicircle production and removal of the vector backbone with this ready-to-add Arabinose Solution—use with SBI's minicircle production strain ZYCY10P3S2T.

The ZYCY10P3S2T Minicircle Producer Strain harbors an arabinose-inducible system to express the PhiC31 integrase and the I-SceI endonuclease simultaneously. The ZYCY10P3S2T strain also contains a robust arabinose transporter LacY A177C gene. Adding arabinose to the media turns on expression of the PhiC31 integrase and endonuclease genes, resulting in separation of the Parental Minicircle Plasmid into the individual minicircle and parental plasmids (from the PhiC31 Integrase activity), and the degradation of the parental plasmid (from I-SceI endonuclease activity).

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