Non-Esterified Fatty Acids (NEFA/FFA) Detection Assay Kit

The RayBiotech Non-Esterified Fatty Acids (NEFA/FFA) Detection Assay Kit provides a simple, reproducible, and sensitive tool for measuring free fatty acids in plasma, serum, cell lysates and other biological liquid samples. This assay uses a coupled enzymatic reaction system (ACS-ACOD Method), first, Acyl CoA Synthetase (ACS) catalyzes fatty acid acylation of coenzyme A. Next, the acyl-CoA product is oxidized by Acyl CoA Oxidase (ACOD), producing hydrogen peroxide which reacts with the Colorimetric Probe to form pink colored product. The optical density at 550nm is directly proportional to the free fatty acid concentration in the sample.