CRISPR Chicken Genome Knockout Library (virus 2 x 10^8 TU)

Product Description

Guide designs for Cellecta’s libraries incorporate state-of-the-art design criteria to ensure the most effective knockout possible. In addition, the region of the sgRNA interacting with the Cas9 nuclease has been optimized for higher efficiency knockout by incorporating the “HEAT” design. It has been shown by Cellecta that specific modifications to the Cas9-binding region of sgRNA significantly improve the effectiveness of gene knockout and that CRISPR libraries containing constructs with these modified sgRNA sequences generate stronger and more robust results than those with the standard sgRNA.

All CRISPR Knockout Pooled Lentiviral sgRNA Libraries are provided with complete sequence information on the vectors, inserts, and guides expressed, as well as Next-Generation Sequencing (NGS) data showing the number of reads per sgRNA as well as the distribution of the sgRNA of the library.