Gibson Assembly Ultra Kit

Simultaneously assemble up to 15 DNA fragments to produce a final product that is several hundred kilobase pairs in length

Product Description

Assembles up to 15 inserts at one time
Simple Two-Step Process - 80 minute reaction with less than 6 minutes hands-on time
Optimal for widely varying fragment sizes from 100 bp-100 kb in length
Complex assemblies of 95% cloning efficiencies
Create constructs up to 1000 kb in length in multi-stage assembly reactions
Assembles without needing restriction enzyme sites
Utilize any vector using simple design strategies
Constructs are joined without the presence of intervening and extraneous sequences
Easily adapted for multiple DNA manipulations, ss oligonucleotides and assembling repetitive sequences
Enhance cloning specificity of PCR products due to homology matching between amplicon and vector
Gibson Assembly method has been cited by more than 1,200 scientific publications since 2009

The Gibson Assembly Ultra method utilizes a special mix of enzymes to assemble DNA quickly and efficiently in a two-step reaction. Multiple DNA fragments can be joined with 95% cloning efficiency and constructs up to 1000 kb in length can be efficiently assembled by designing multi-stage Gibson Assembly Ultra reactions.

Applications

Clone multiple inserts into a vector without relying on the availability of restriction sites
Large Fragment Assemblies – Assemble gene clusters and genome-size fragments
Site-directed mutagenesis to make simultaneous changes in a single reaction

How It Works

The Gibson Assembly Ultra method utilizes a special mix of enzymes to assemble DNA quickly and efficiently in a two-step reaction. Multiple DNA fragments can be joined with 95% cloning efficiency. Constructs up to 1000 kb in length can be efficiently assembled by designing multi-stage Gibson Assembly Ultra reactions.

Design DNA fragments with overlap regions
Combine the fragments and Ultra Master Mix A => Master Mix A catalyzes 3’ chew back
Inactivate Ultra Master Mix A at 75°C
Anneal DNA fragments at 60°C
Combine the fragments and Ultra Master Mix B >> Master Mix B catalyzes repair

The resulting construct is double stranded, fully sealed, and can be used immediately for downstream molecular biology applications

Figure 1: The Gibson Assembly Ultra Kit workflow

For less complex constructs

An assembly of up to 5 fragments in a single step can be performed with the Gibson Assembly HIFI Kit.

Primer Design Tool

To assist you in designing primers for the assembly of DNA fragments the Gibson Assembly Primer Design Tool has been developed.

Commercial Use Rights

All Gibson Assembly^® products purchased from BioCat as Codex DNA authorized distributor include full commercial use rights. Please contact us in case further details are needed.